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Journal: Veterinary Sciences
Article Title: Detection and Phylogenetic Analysis of Caprine Arthritis Encephalitis Virus Using TaqMan-based qPCR in Eastern China
doi: 10.3390/vetsci11030138
Figure Lengend Snippet: Phylogenetic tree of caprine arthritis encephalitis virus (CAEV) based on the partial gag gene (about 571 bp). The three strains isolated in this study—JS-2023-1 (no. PP382771), JS-2023-2 (no. PP382772), and SH-2023 (no. PP382773)—are marked by red circles. The other 28 representative sequences were downloaded from GenBank and edited to obtain the corresponding segments using Editseq version 7.1.0 software (DNASTAR Inc., Madison, WI, USA; Lole et al., 1999) based on the sequencing primers used. Multiple sequence alignments were performed using the Clustal V method, and a phylogenetic analysis of the recombinants was performed using MegAlign version 7.1.0 software (DNASTAR Inc., Madison, WI, USA; Lole et al., 1999).
Article Snippet: To construct primers and probes, the nucleotide sequences of the whole genomes of CAEV were retrieved from the GenBank database and compared, using the CLUSTAL V algorithm in the
Techniques: Virus, Isolation, Software, Sequencing